外源褪黑激素促进绒山羊皮肤毛囊相关基因表达差异的研究

doi:10.11843/j.issn.0366-6964.2016.11.008

Abstract

Abstract:

Effects of embedding melatonin for Arbas Cashmere goats in telogen phase on control of cashmere growth cycle were conducted in order to extend anagen phase and increase the cashmere production. Cashmere goats were selected and randomly divided into 2 groups,each group with 3 goats（ the experiment group (T1,T2,T3) and the control group (C1,C2,C3)）. Skin tissues of the treatment and control groups were taken and tissue sections were observed for the influence of embedding melatonin on the induction of hair follicle growth. The total RNA was extracted from the 2 groups of samples and synthesized marked cDNA probes by reverse transcription, and had microarray hybridization on 8×15 K(Agilent) sheep genome-wide expression profile to screen differentially expressed genes. The results were verified by real-time fluorescent quantitative PCR technology. Histological analysis showed that embedding melatonin could obviously promote hair follicle growth. Microarray data showed that there were 95 differently expressed genes, including 61 up-regulated and 34 down-regulated. GO analysis indicated that the genes of 47.78% participated in the molecular function, those of 33.89% were involved in biological processes, and those of 18.33% were associated with the composition of cell. Compared with the control groups, differential expression of related genes in treatment groups were involved in the hair follicle morphogenesis and biological processes of surrounding skin appendages. These differently expressed genes provide valuable reference to research Cashmere goat hair follicle growth and the function of the cyclical controlling genes.

CLC Number:

S827.2

LIU Bin，YANG Jun，A Yun-ga，WU Ri-gexilatu，NA Shun-qimuge，HAO Ba-yasihuliang，ZHAO Cun-fa，LI Yu-rong. Exogenous Melatonin Promotes Expression Differences of Skin Hair Follicles Related Genes in Cashmere Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2210-2217.

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Exogenous Melatonin Promotes Expression Differences of Skin Hair Follicles Related Genes in Cashmere Goat

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